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IL-1離體肺灌注系統(tǒng)的概述

HSE—HA生理學(xué)系統(tǒng)是一系列應(yīng)用廣泛的完備產(chǎn)品，可用于整體動物生理學(xué)、立體或在體器官和組織灌注及組織浴槽研究。這些系統(tǒng)具有廣泛的應(yīng)用，包括傳統(tǒng)的安全藥理學(xué)、DMPK、毒理學(xué)、呼吸系統(tǒng)、心臟病學(xué)、分子生物學(xué)和生理學(xué)。每個系統(tǒng)都設(shè)計了標(biāo)準(zhǔn)的組件，再加一些其他配置以適合特殊研究的需要。。標(biāo)注組件也意味著如果你是需要將現(xiàn)有設(shè)備配齊，或者進(jìn)行系統(tǒng)升級，或者是完整的配套產(chǎn)品，你都只需要購買需要的構(gòu)件。HSE-HA生理學(xué)系統(tǒng)為你提供各種工具，使你的研究走向多產(chǎn)和成功。

我們提供大多數(shù)生理學(xué)檢測設(shè)備，包括放大器、電極、傳感器和電化學(xué)檢測器。參數(shù)包括力、位移、壓力、液體和氣體流、心電圖（單導(dǎo)聯(lián)—12導(dǎo)統(tǒng)）、MAP（單個或多個部位）、腦電圖、*、連續(xù)支流灌注傳統(tǒng)的血?dú)鈪?shù)和電解質(zhì)的分析，可以選擇程序化軟件和傳統(tǒng)的刺激器作為放大器、電極和傳感器等產(chǎn)品的補(bǔ)充。

HSE-DAQ系統(tǒng)產(chǎn)品家族（BDAS、ACAD、HAEMODYN、ISOHEART、PULMODYN、PENNOCK）可以記錄你的初始信號，計算到處的參數(shù)，注釋數(shù)據(jù)，快速簡易的輸入確定的數(shù)據(jù)用于分析。同時還可以選擇用于進(jìn)一步分析的軟件模塊，帶狀記錄紙打印，自動和控制模塊等。產(chǎn)品經(jīng)過預(yù)先設(shè)置，打開包裝即可使用，并專門設(shè)計符合你的系統(tǒng)配置和數(shù)據(jù)分析要求。

• Investigation of ventilation and perfusion in the isolated mouse lung

• Non-damaging in-situ preparation

• Negative-pressure ventilation similar to in-vivo condition

• Regular induction of deep inspiration （sigh, augmented breath）

• Pneumotachometer and air humidifier with small dead volume

• Continuous measurement of respiratory mechanics （tidal volume, airway resistance, dynamic compliance） and perfusate characteristics （pressure, lung vascular resistance, pO2, pH）

Most of the studies with isolated perfused lungs have been performed with lungs obtained from rats, guinea pigs, rabbits or dogs. The interaction of the immune system with the lung is currently an area of great interest. The mouse is the best characterized rodent species with respect to its immune system. Immunologic probes such as antibodies and cytokines are more widely available for mice versus other species. To facilitate studies on the interaction of the lung and the immune system, an apparatus for perfusing and ventilating mouse lungs has been developed. This apparatus is also suitable for studying the respiratory system of knock-out mice.

Ventilation

The lung is placed in the artificial thoracic chamber and ventilated at negative pressure. It is connected through the tracheal cannula to the pneumotachometer for measurement of respiratory flow. The respiration rate, the inspiratory and the end-expiratory pressure can be adjusted by separate controls （Positive ventilation using a regular Minivent ventilator is also possible）.

Perfusion

The pulmonary artery is cannulated to provide perfusion. The perfusate is passed by means of a roller pump at constant flow through the heat exchanger, through a bubble trap to the pulmonary artery and finally into the lung vascular bed. The perfusate outflow is usually provided by cannulating the left atrium of the heart.