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其他離體器官灌注系統(tǒng)

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更新時(shí)間:2022-05-28 22:20:02瀏覽次數(shù):727次

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用于豬的離體肝臟或腎臟的灌注系統(tǒng)

IPL&K其他離體器官灌注系統(tǒng)的概述

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• for use in physiological or pharmacological research for the perfusion of a pig liver or kidney with blood or erythrocyte containing perfusate

 

basic system

the pig liver to be perfused is placed in a moist, thermostated chamber and perfused with blood through the portal vein under constant-flow conditions. a centrifugal pump with a gentle action on blood is employed to reduce hemolysis; it is controlled by an electronic controller (scp) so that the apparatus can be operated under constant-flow conditions. the system can also operate under constant-pressure conditions. flow control requires that the flow is measured by means of a flow probe (16n) and a flowmeter (ttfm).

the pressure should also be measured to monitor the experiment. this requires a pressure transducer (p75) and a bridge amplifier (tam). a suitable oxygenator is employed for oxygenating and warming the blood; it can be of a type used in human medicine, e.g. terumo capiox sx10®.

components required

the following items are required for operating the apparatus:

• thermostated moist chamber type 869/2

• circulating thermostat of approx. 3 liter capacity, distilled water and an anti-algal agent, e.g. thermoklar

• pump with electrical control. for blood we recommend a pump drive bvp-zx with centrifugal pump head, see the pump section a

• servo controller for perfusion (scp) type 704, see page k12

• flowmeter (ttfm) with flow probe (16n), see pages j6 and j7

• recommended: pressure transducer (p75), see page j3 and bridge amplifier (tam-d), section i of this catalog

• oxygenator with heat exchanger, e.g. terumo capiox sx10®

• source of carbogen for aeration. central supply or carbogen cylinder with pressure reducing valve

additional monitoring equipment can be added to the system as required to cr-eate a custom, application specific, turn key system for your unique research needs.

 

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