植物25羥基維生素D3(25(OH)D3/25 HVD3)ELISA Kit |
保存條件:2-8℃
用 途:主要用于實(shí)驗(yàn)室科研實(shí)驗(yàn)。不得用于臨床使用。
保質(zhì)期:6個(gè)月。
植物25羥基維生素D3(25(OH)D3/25 HVD3)ELISA Kit |
植物25羥基維生素D3(25(OH)D3/25 HVD3)ELISA Kit |
以及ELISA試劑盒使用發(fā)表的文章等。請我公司的銷售技術(shù)人員。
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標(biāo)本的采集及保存
1.血清:全血標(biāo)本請于室溫放置2小時(shí)或4℃過夜后于1000g離心20分鐘,取上清即可檢測,或?qū)?biāo)本放于-20℃或-80℃保存,但應(yīng)避免反復(fù)凍融。
2.血漿:可用EDTA或肝素作為抗凝劑,標(biāo)本采集后30分鐘內(nèi)于2 - 8°C 1000 g離心15分鐘,或?qū)?biāo)本放于-20℃或-80℃保存,但應(yīng)避免反復(fù)凍融。
3.細(xì)胞培養(yǎng)物上清或其它生物標(biāo)本:1000g離心20分鐘,取上清即可檢測,或?qū)?biāo)本放于-20℃或-80℃保存,但應(yīng)避免反復(fù)凍融。
檢測方法:ELISA酶聯(lián)免疫(雙抗夾心法)
檢測標(biāo)本:血清、血漿、組織、細(xì)胞上清液、尿液等。
ELISA試劑盒的組成:
1.30倍濃縮洗滌液 20ml×1瓶 7.顯色劑B液 6ml×1/瓶
2.酶標(biāo)試劑 6ml×1瓶 8.標(biāo)準(zhǔn)品(96μg/L) 0.5ml×1瓶
3.酶標(biāo)包被板 12孔×8條 9.標(biāo)準(zhǔn)品稀釋液 1.5ml×1瓶
4.樣品稀釋液 6ml×1瓶 10.說明書 1份
5.顯色劑A液 6ml×1瓶 11.封板膜 2張
6.顯色劑B液 6ml×1/瓶 12.密封袋 1個(gè)
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操作注意事項(xiàng):
1.試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存。
2.濃洗滌液可能會有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果。
3.各步加樣均應(yīng)使用加樣器,并經(jīng)常校對其準(zhǔn)確性,以避免試驗(yàn)誤差。一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,*使用排槍加樣。
4.請每次測定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔。如標(biāo)本中待測物質(zhì)含量過高(樣本OD值大于標(biāo)準(zhǔn)品孔*孔的OD值),請先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測定,計(jì)算時(shí)請zui后乘以總稀釋倍數(shù)(×n×5)。
5.封板膜只限一次性使用,以避免交叉污染。
6.底物請避光保存。
7.嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).
8.所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。
9.本試劑不同批號組分不得混用。
10. 如與英文說明書有異,以英文說明書為準(zhǔn)。
所有評論僅代表網(wǎng)友意見,與本站立場無關(guān)。