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包被用人谷氨酸脫氫酶(GDH/GLDH)ELISA試劑盒抗原:用于包被固相載體的抗原按其來源不同可分為天然抗原、重組抗原和合成多肽抗原三大類。天然抗原可取自動物組織、微生物培養(yǎng)物等,須經(jīng)提取純化才能作包被用。
包被用抗體:包被固相載體的抗體應(yīng)具有高親和力和高特異性,可取材于抗血清或含單克隆抗體的腹水或培養(yǎng)液。如免疫用抗原中含有雜質(zhì)(即便是極微量的),在抗血清中將出現(xiàn)雜抗體,必須除去(可用吸收法)后才能用于ELISA,以保證試驗的特異性??寡宀荒苤苯佑糜诎唬瑧?yīng)先提取IgG,通常采用硫酸銨鹽析和Sephadex凝膠過濾法。一般經(jīng)硫酸銨鹽析粗提的IgG已可用于包被,高度純化的IgG性質(zhì)不穩(wěn)定。如需用高親和力的抗體包被以提高試驗的敏感性,則可采用親和層析法以除去抗血清中含量較多的非特異性IgG。腹水中單抗的濃度較高,特異性亦較強,因此不需要作吸收和親和層析處理,一般可將腹水作適當(dāng)稀釋后直接包被,必要時也可用純化的IgG。應(yīng)用單抗包被時應(yīng)注意,一種單抗僅針對一種抗原決定簇,在某些情況下,用多種單抗混合包被,可取得更好的效果。
112791 小白蛋白(PVALB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Parvalbumin (PVALB) 48T/96T Rattus norvegicus (Rat)
112792 信號素3E(SEMA3E)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Semaphorin 3E (SEMA3E) 48T/96T Mus musculus (Mouse)
112793 CD276分子(CD276)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Cluster Of Differentiation 276 (CD276) 48T/96T Homo sapiens (Human)
112794 淋巴細胞抗原75(LY75)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Lymphocyte Antigen 75 (LY75) 48T/96T Mus musculus (Mouse)
112795 前妊娠蛋白關(guān)聯(lián)子宮內(nèi)膜蛋白(PAEP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Progestagen Associated Endometrial Protein (PAEP) 48T/96T Homo sapiens (Human)
112796 EB病毒誘導(dǎo)蛋白3(EBI3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Epstein Barr Virus Induced Protein 3 (EBI3) 48T/96T Mus musculus (Mouse)
112797 肝配蛋白A4(EFNA4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Ephrin A4 (EFNA4) 48T/96T Homo sapiens (Human)
112798 微小染色體維持缺陷蛋白5(MCM5)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Minichromosome Maintenance Deficient 5 (MCM5) 48T/96T Homo sapiens (Human)
112799 Zeste同源物增強子2(EZH2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Enhancer Of Zeste Homolog 2 (EZH2) 48T/96T Homo sapiens (Human)
112800 幾丁質(zhì)酶1(CHIT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Chitinase 1 (CHIT1) 48T/96T Mus musculus (Mouse)
112801 乳腺絲抑蛋白(Maspin)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Maspin 48T/96T Homo sapiens (Human)
112802 骨成型蛋白受體1B(BMPR1B)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Bone Morphogenetic Protein Receptor 1B (BMPR1B) 48T/96T Homo sapiens (Human)
112803 Apelin受體(APLNR)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Apelin Receptor (APLNR) 48T/96T Rattus norvegicus (Rat)
112804 90kDa熱休克蛋白αA1(HSP90αA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Heat Shock Protein 90kDa Alpha A1 (HSP90aA1) 48T/96T Mus musculus (Mouse)
112805 谷胱甘肽S轉(zhuǎn)移酶π1(GSTπ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Glutathione S Transferase Pi (GSTp) 48T/96T Sus scrofa; Porcine (Pig)
112806 β-位淀粉樣前體蛋白裂解酶1(βACE1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Beta-Site APP Cleaving Enzyme 1 (bACE1) 48T/96T Mus musculus (Mouse)
112807 血管內(nèi)皮鈣黏蛋白(CDH5)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Cadherin 5 (CDH5) 48T/96T Mus musculus (Mouse)
112808 信號素3F(SEMA3F)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Semaphorin 3F (SEMA3F) 48T/96T Homo sapiens (Human)
112809 亨廷頓蛋白(HTT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Huntingtin (HTT) 48T/96T Rattus norvegicus (Rat)
112810 亨廷頓蛋白(HTT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Huntingtin (HTT) 48T/96T Mus musculus (Mouse)
112811 蝰蛇毒素(Viperin)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Viperin 48T/96T Homo sapiens (Human)
112812 神經(jīng)元衍生孤兒受體1()檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Neuron Derived Orphan Receptor 1 () 48T/96T Rattus norvegicus (Rat)
112813 前折疊蛋白亞基1(PFDN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Prefoldin Subunit 1 (PFDN1) 48T/96T Homo sapiens (Human)
112814 不均一核核糖核蛋白U(HNRPU)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Heterogeneous Nuclear Ribonucleoprotein U (HNRPU) 48T/96T Homo sapiens (Human)
112815 含V-Set域T細胞激活抑制因子1(VTCN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for V-Set Domain Containing T-Cell Activation Inhibitor 1 (VTCN1) 48T/96T Homo sapiens (Human)
112816 趨化因子C-X-C-基元受體4(CXCR4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Chemokine C-X-C-Motif Receptor 4 (CXCR4) 48T/96T Homo sapiens (Human)
112817 Enah/Vasp樣蛋白(EVL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Enah/Vasp Like Protein (EVL) 48T/96T Homo sapiens (Human)
112818 剪切多聚腺苷酸化特異性因子1(CPSF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Cleavage And Polyadenylation Specific Factor 1 (CPSF1) 48T/96T Homo sapiens (Human)
112819 雙特異性酪氨酸磷酸化調(diào)節(jié)激酶1A(DYRK1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) 48T/96T Mus musculus (Mouse)
112820 ⅩⅣ型膠原(COL14)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Collagen Type XIV (COL14) 48T/96T Homo sapiens (Human)
112821 角蛋白4(KRT4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Keratin 4 (KRT4) 48T/96T Homo sapiens (Human)
112822 補體因子H相關(guān)蛋白3(CFHR3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Complement Factor H Related Protein 3 (CFHR3) 48T/96T Homo sapiens (Human)
112823 Smad同源物3(Smad3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Mothers Against Decapentaplegic Homolog 3 (Smad3) 48T/96T Rattus norvegicus (Rat)
112824 含CUB域蛋白1(CDCP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for CUB Domain Containing Protein 1 (CDCP1) 48T/96T Homo sapiens (Human)
112825 δ樣蛋白4(δLL4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Delta Like Protein 4 (dLL4) 48T/96T Homo sapiens (Human)
112826 肌營養(yǎng)不良蛋白關(guān)聯(lián)糖蛋白1(DAG1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Dystrophin Associated Glycoprotein 1 (DAG1) 48T/96T Mus musculus (Mouse)
112827 血小板反應(yīng)蛋白解整合素金屬肽酶4(ADAMTS4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4) 48T/96T Homo sapiens (Human)
112828 絲聚蛋白(FLG)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Filaggrin (FLG) 48T/96T Homo sapiens (Human)
112829 脯氨酸/精氨酸豐富端亮氨酸豐富重復(fù)蛋白(PRELP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Proline Arginine Rich End Leucine Rich Repeat Protein (PRELP) 48T/96T Homo sapiens (Human)
112830 透明質(zhì)酸合酶2(HAS2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Hyaluronan Synthase 2 (HAS2) 48T/96T Homo sapiens (Human)
112831 碳酸酐酶Ⅵ(CA6)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Carbonic Anhydrase VI (CA6) 48T/96T Homo sapiens (Human)
112832 嵌套蛋白(NES)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Nestin (NES) 48T/96T Homo sapiens (Human)
112833 成纖維細胞生長因子5(FGF5)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Fibroblast Growth Factor 5 (FGF5) 48T/96T Homo sapiens (Human)
112834 高爾基體蛋白A8家族成員A(GOLGA8A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Golgin A8 Family, Member A (GOLGA8A) 48T/96T Homo sapiens (Human)
112835 戴帽蛋白肌Z系β(CAPZβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Capping Protein Muscle Z Line Beta (CAPZb) 48T/96T Homo sapiens (Human)
112836 核纖層蛋白B1(LMNB1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Lamin B1 (LMNB1) 48T/96T Homo sapiens (Human)
112837 熱休克蛋白47(HSP47)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Heat Shock Protein 47 (HSP47) 48T/96T Homo sapiens (Human)
112838 含卷曲螺旋域蛋白80(CCDC80)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Coiled Coil Domain Containing Protein 80 (CCDC80) 48T/96T Homo sapiens (Human)
112839 圍脂滴蛋白4(PLIN4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Perilipin 4 (PLIN4) 48T/96T Mus musculus (Mouse)
112840 溶血磷脂酸受體1(LPAR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Lysophosphatidic Acid Receptor 1 (LPAR1) 48T/96T Homo sapiens (Human)
112841 微粒體甘油三酸酯轉(zhuǎn)運蛋白(MTTP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Microsomal Triglyceride Transfer Protein (MTTP) 48T/96T Mus musculus (Mouse)
112842 維生素D受體(VDR)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Vitamin D Receptor (VDR) 48T/96T Mus musculus (Mouse)
112843 銅轉(zhuǎn)運蛋白1(COPT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Copper Transporter 1 (COPT1) 48T/96T Homo sapiens (Human)
112844 微精蛋白β(MSMβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Microseminoprotein Beta (MSMb) 48T/96T Homo sapiens (Human)
112845 弗林蛋白酶(FUR)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Furin (FUR) 48T/96T Homo sapiens (Human)
112846 細胞色素P450氧化還原酶(CPR)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Cytochrome P450 Reductase (CPR) 48T/96T Homo sapiens (Human)
112847 過氧化氫酶(CAT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Catalase (CAT) 48T/96T Mus musculus (Mouse)
112848 轉(zhuǎn)膠蛋白(TAGLN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Transgelin (TAGLN) 48T/96T Rattus norvegicus (Rat)
112849 左右決定因子1(LEFTY1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Left/Right Determination Factor 1 (LEFTY1) 48T/96T Homo sapiens (Human)
112850 微纖絲關(guān)聯(lián)蛋白4(MFAP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Microfibrillar Associated Protein 4 (MFAP4) 48T/96T Rattus norvegicus (Rat)
將抗原或抗體固定在過程稱為包被(coating)。換言之,包被即是抗原或抗體結(jié)合到固相載體表面的過程,脂類物質(zhì)無法與固相載體結(jié)合,可將其在有機溶劑(例如乙醇)中溶解后加入板孔中,開蓋置冰箱過夜或冷風(fēng)吹干,待酒精揮發(fā)后,讓脂質(zhì)自然干固在固相表面。抗心磷脂抗體的ELISA試劑一般采用這種包被方式。
人谷氨酸脫氫酶(GDH/GLDH)ELISA試劑盒
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