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包被用人膽囊收縮素/腸促胰酶肽(CCK)ELISA試劑盒抗原:用于包被固相載體的抗原按其來源不同可分為天然抗原、重組抗原和合成多肽抗原三大類。天然抗原可取自動物組織、微生物培養(yǎng)物等,須經(jīng)提取純化才能作包被用。
包被用抗體:包被固相載體的抗體應具有高親和力和高特異性,可取材于抗血清或含單克隆抗體的腹水或培養(yǎng)液。如免疫用抗原中含有雜質(即便是極微量的),在抗血清中將出現(xiàn)雜抗體,必須除去(可用吸收法)后才能用于ELISA,以保證試驗的特異性??寡宀荒苤苯佑糜诎?,應先提取IgG,通常采用硫酸銨鹽析和Sephadex凝膠過濾法。一般經(jīng)硫酸銨鹽析粗提的IgG已可用于包被,高度純化的IgG性質不穩(wěn)定。如需用高親和力的抗體包被以提高試驗的敏感性,則可采用親和層析法以除去抗血清中含量較多的非特異性IgG。腹水中單抗的濃度較高,特異性亦較強,因此不需要作吸收和親和層析處理,一般可將腹水作適當稀釋后直接包被,必要時也可用純化的IgG。應用單抗包被時應注意,一種單抗僅針對一種抗原決定簇,在某些情況下,用多種單抗混合包被,可取得更好的效果。
113631 Persephin蛋白(PSPN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Persephin (PSPN) 48T/96T Homo sapiens (Human)
113632 Ⅹ型膠原(COL10)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Collagen Type X (COL10) 48T/96T Rattus norvegicus (Rat)
113633 Ⅸ型膠原α1(COL9α1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Collagen Type IX Alpha 1 (COL9a1) 48T/96T Rattus norvegicus (Rat)
113634 存活素(Surv)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Survivin (Surv) 48T/96T Homo sapiens (Human)
113635 整合素αM(ITGαM)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Integrin Alpha M (ITGaM) 48T/96T Mus musculus (Mouse)
113636 前梯度蛋白2(AGR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Anterior Gradient Protein 2 (AGR2) 48T/96T Homo sapiens (Human)
113637 平足蛋白(PDPN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Podoplanin (PDPN) 48T/96T Homo sapiens (Human)
113638 Ⅲ型前膠原(PCⅢ)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Procollagen III (PCIII) 48T/96T Rattus norvegicus (Rat)
113639 層粘連蛋白(LN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Laminin (LN) 48T/96T Rattus norvegicus (Rat)
113640 阻抑素(PHB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Prohibitin (PHB) 48T/96T Mus musculus (Mouse)
113641 細胞間粘附分子4(ICAM4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Intercellular Adhesion Molecule 4 (ICAM4) 48T/96T Homo sapiens (Human)
113642 B-細胞激活因子(BAFF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for B-Cell Activating Factor (BAFF) 48T/96T Mus musculus (Mouse)
113643 聚集蛋白聚糖(AGC)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Aggrecan (AGC) 48T/96T Oryctolagus cuniculus (Rabbit)
113644 葉酸受體1(FOLR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Folate Receptor 1, Adult (FOLR1) 48T/96T Homo sapiens (Human)
113645 拓撲異構酶Ⅰ(*)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Topoisomerase I (*) 48T/96T Homo sapiens (Human)
113646 Ⅹ型膠原(COL10)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Collagen Type X (COL10) 48T/96T Homo sapiens (Human)
113647 畸胎瘤衍生生長因子1(TDGF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Teratocarcinoma Derived Growth Factor 1 (TDGF1) 48T/96T Homo sapiens (Human)
113648 跨膜絲氨酸蛋白酶2(TMPRSS2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Transmembrane Protease, Serine 2 (TMPRSS2) 48T/96T Homo sapiens (Human)
113649 膜聯(lián)蛋白A6(ANXA6)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Annexin A6 (ANXA6) 48T/96T Homo sapiens (Human)
113650 補體3a(C3a)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Complement Component 3a (C3a) 48T/96T Canis familiaris; Canine (Dog)
113651 腫瘤壞死因子配體超家族成員12(TNFSF12)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Tumor Necrosis Factor Ligand Superfamily, Member 12 (TNFSF12) 48T/96T Rattus norvegicus (Rat)
113652 皮質醇結合球蛋白(CBG)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Corticosteroid Binding Globulin (CBG) 48T/96T Homo sapiens (Human)
113653 神經(jīng)元特異性烯醇化酶(NSE)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Enolase, Neuron Specific (NSE) 48T/96T Oryctolagus cuniculus (Rabbit)
113654 牙本質涎磷蛋白(DSPP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Dentin Sialophosphoprotein (DSPP) 48T/96T Homo sapiens (Human)
113655 纖維蛋白原(FG)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Fibrinogen (FG) 48T/96T Bos taurus; Bovine (Cattle)
113656 衰老關鍵蛋白5(FBLN5)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Fibulin 5 (FBLN5) 48T/96T Homo sapiens (Human)
113657 脯氨酰羧肽酶(PRCP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Prolylcarboxypeptidase (PRCP) 48T/96T Rattus norvegicus (Rat)
113658 蛋白C抑制因子(PCI)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Protein C Inhibitor (PCI) 48T/96T Homo sapiens (Human)
113659 髓過氧化物酶(MPO)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Myeloperoxidase (MPO) 48T/96T Equus caballus; Equine (Horse)
113660 髓過氧化物酶(MPO)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Myeloperoxidase (MPO) 48T/96T Bos taurus; Bovine (Cattle)
113661 乳鐵傳遞蛋白(LTF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Lactoferrin (LTF) 48T/96T Capra hircus; Caprine (Goat)
113662 乳鐵傳遞蛋白(LTF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Lactoferrin (LTF) 48T/96T Canis familiaris; Canine (Dog)
113663 乳鐵傳遞蛋白(LTF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Lactoferrin (LTF) 48T/96T Equus caballus; Equine (Horse)
113664 補體成分5a(C5a)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Complement Component 5a (C5a) 48T/96T Oryctolagus cuniculus (Rabbit)
113665 妊娠相關血漿蛋白A2(PAPPA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Pappalysin 2 (PAPPA2) 48T/96T Homo sapiens (Human)
113666 內(nèi)皮素受體A(ETRA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Endothelin Receptor A (ETRA) 48T/96T Homo sapiens (Human)
113667 谷胱甘肽還原酶(GR)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Glutathione Reductase (GR) 48T/96T Rattus norvegicus (Rat)
113668 阿黑皮素原(POMC)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Proopiomelanocortin (POMC) 48T/96T Mus musculus (Mouse)
113669 雄激素受體(AR)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Androgen Receptor (AR) 48T/96T Rattus norvegicus (Rat)
113670 Endoglin蛋白(ENG)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Endoglin (ENG) 48T/96T Rattus norvegicus (Rat)
113671 層粘連蛋白(LN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Laminin (LN) 48T/96T Homo sapiens (Human)
113672 誘導型一氧化氮合酶(NOS2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Nitric Oxide Synthase 2, Inducible (NOS2) 48T/96T Canis familiaris; Canine (Dog)
113673 緊密連接蛋白1(TJP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Tight Junction Protein 1 (TJP1) 48T/96T Homo sapiens (Human)
113674 干擾素誘導T-細胞α亞族趨化劑(ITαC)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) 48T/96T Rattus norvegicus (Rat)
113675 干擾素γ誘導單核因子(MIγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Monokine Induced By Interferon Gamma (MIg) 48T/96T Rattus norvegicus (Rat)
113676 α1-抗胰蛋白酶(α1AT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Alpha-1-Antitrypsin (a1AT) 48T/96T Rattus norvegicus (Rat)
113677 穿孔素1(PRF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Perforin 1 (PRF1) 48T/96T Rattus norvegicus (Rat)
113678 胰多肽(PP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Pancreatic Polypeptide (PP) 48T/96T Mus musculus (Mouse)
113679 簇集蛋白(CLU)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Clusterin (CLU) 48T/96T Canis familiaris; Canine (Dog)
113680 腫瘤壞死因子相關凋亡誘導配體(TRAIL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) 48T/96T Rattus norvegicus (Rat)
113681 腫瘤壞死因子相關凋亡誘導配體(TRAIL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) 48T/96T Canis familiaris; Canine (Dog)
113682 末端補體復合體C5b-9(C5b-9)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Terminal Complement Complex C5b-9 (C5b-9) 48T/96T Homo sapiens (Human)
113683 精氨酸(Arg)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Arginine (Arg) 48T/96T General
113684 甘露聚糖結合凝集素關聯(lián)絲氨酸蛋白酶2(MASP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Mannose Associated Serine Protease 2 (MASP2) 48T/96T Homo sapiens (Human)
113685 纖維膠凝蛋白1(FCN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Ficolin 1 (FCN1) 48T/96T Homo sapiens (Human)
113686 α2-巨球蛋白(α2M)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Alpha-2-Macroglobulin (a2M) 48T/96T Rattus norvegicus (Rat)
113687 胃動蛋白1(GKN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Gastrokine 1 (GKN1) 48T/96T Homo sapiens (Human)
113688 促卵泡素(FSH)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Follicle Stimulating Hormone (FSH) 48T/96T Mus musculus (Mouse)
113689 血管內(nèi)皮生長因子165(VEGF165)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Vascular Endothelial Growth Factor 165 (VEGF165) 48T/96T Bos taurus; Bovine (Cattle)
113690 橋粒斑蛋白(DSP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Desmoplakin (DSP) 48T/96T Homo sapiens (Human)
將抗原或抗體固定在過程稱為包被(coating)。換言之,包被即是抗原或抗體結合到固相載體表面的過程,脂類物質無法與固相載體結合,可將其在有機溶劑(例如乙醇)中溶解后加入板孔中,開蓋置冰箱過夜或冷風吹干,待酒精揮發(fā)后,讓脂質自然干固在固相表面??剐牧字贵w的ELISA試劑一般采用這種包被方式。
人膽囊收縮素/腸促胰酶肽(CCK)ELISA試劑盒
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