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包被用人腮腺炎病毒IgM ELISA試劑盒抗原:用于包被固相載體的抗原按其來源不同可分為天然抗原、重組抗原和合成多肽抗原三大類。天然抗原可取自動物組織、微生物培養(yǎng)物等,須經(jīng)提取純化才能作包被用。
包被用抗體:包被固相載體的抗體應具有高親和力和高特異性,可取材于抗血清或含單克隆抗體的腹水或培養(yǎng)液。如免疫用抗原中含有雜質(zhì)(即便是極微量的),在抗血清中將出現(xiàn)雜抗體,必須除去(可用吸收法)后才能用于ELISA,以保證試驗的特異性??寡宀荒苤苯佑糜诎?,應先提取IgG,通常采用硫酸銨鹽析和Sephadex凝膠過濾法。一般經(jīng)硫酸銨鹽析粗提的IgG已可用于包被,高度純化的IgG性質(zhì)不穩(wěn)定。如需用高親和力的抗體包被以提高試驗的敏感性,則可采用親和層析法以除去抗血清中含量較多的非特異性IgG。腹水中單抗的濃度較高,特異性亦較強,因此不需要作吸收和親和層析處理,一般可將腹水作適當稀釋后直接包被,必要時也可用純化的IgG。應用單抗包被時應注意,一種單抗僅針對一種抗原決定簇,在某些情況下,用多種單抗混合包被,可取得更好的效果。
117181 干擾素α(IFNα)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Interferon Alpha (IFNa) 48T/96T Mus musculus (Mouse)
117182 凝血因子Ⅱ(F2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Coagulation Factor II (F2) 48T/96T Homo sapiens (Human)
117183 過氧化物酶體增殖物激活受體γ(PPARγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Peroxisome Proliferator Activated Receptor Gamma (PPARg) 48T/96T Mus musculus (Mouse)
117184 過氧化物酶體增殖物激活受體γ(PPARγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Peroxisome Proliferator Activated Receptor Gamma (PPARg) 48T/96T Rattus norvegicus (Rat)
117185 過氧化物酶體增殖物激活受體γ(PPARγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Peroxisome Proliferator Activated Receptor Gamma (PPARg) 48T/96T Homo sapiens (Human)
117186 C反應蛋白(CRP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for C Reactive Protein (CRP) 48T/96T Mus musculus (Mouse)
117187 C反應蛋白(CRP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for C Reactive Protein (CRP) 48T/96T Rattus norvegicus (Rat)
117188 神經(jīng)介素B(NMB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Neuromedin B (NMB) 48T/96T Rattus norvegicus (Rat)
117189 神經(jīng)介素B(NMB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Neuromedin B (NMB) 48T/96T Mus musculus (Mouse)
117190 神經(jīng)介素B(NMB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Neuromedin B (NMB) 48T/96T Homo sapiens (Human)
117191 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Glial Fibrillary Acidic Protein (GFAP) 48T/96T Mus musculus (Mouse)
117192 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Glial Fibrillary Acidic Protein (GFAP) 48T/96T Rattus norvegicus (Rat)
117193 神經(jīng)膠質(zhì)纖維酸性蛋白(GFAP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Glial Fibrillary Acidic Protein (GFAP) 48T/96T Homo sapiens (Human)
117194 多功能肽酶2(LMP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Large Multifunctional Peptidase 2 (LMP2) 48T/96T Mus musculus (Mouse)
117195 多功能肽酶2(LMP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Large Multifunctional Peptidase 2 (LMP2) 48T/96T Rattus norvegicus (Rat)
117196 花生四烯酸-15-脂加氧酶(ALOX15)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Arachidonate-15-Lipoxygenase (ALOX15) 48T/96T Mus musculus (Mouse)
117197 花生四烯酸-15-脂加氧酶(ALOX15)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Arachidonate-15-Lipoxygenase (ALOX15) 48T/96T Rattus norvegicus (Rat)
117198 花生四烯酸-15-脂加氧酶(ALOX15)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Arachidonate-15-Lipoxygenase (ALOX15) 48T/96T Homo sapiens (Human)
117199 血清淀粉樣蛋白A(SAA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Serum Amyloid A (SAA) 48T/96T Bos taurus; Bovine (Cattle)
117200 血清淀粉樣蛋白A(SAA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Serum Amyloid A (SAA) 48T/96T Mus musculus (Mouse)
117201 血清淀粉樣蛋白A(SAA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Serum Amyloid A (SAA) 48T/96T Homo sapiens (Human)
117202 結合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Haptoglobin (Hpt) 48T/96T Rattus norvegicus (Rat)
117203 結合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Haptoglobin (Hpt) 48T/96T Mus musculus (Mouse)
117204 結合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Haptoglobin (Hpt) 48T/96T Bos taurus; Bovine (Cattle)
117205 結合珠蛋白(Hpt)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Haptoglobin (Hpt) 48T/96T Homo sapiens (Human)
117206 銅藍蛋白(CP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Ceruloplasmin (CP) 48T/96T Rattus norvegicus (Rat)
117207 α1-酸性糖蛋白(α1AGP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP) 48T/96T Mus musculus (Mouse)
117208 α1-酸性糖蛋白(α1AGP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP) 48T/96T Rattus norvegicus (Rat)
117209 α1-酸性糖蛋白(α1AGP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP) 48T/96T Bos taurus; Bovine (Cattle)
117210 α1-酸性糖蛋白(α1AGP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Alpha-1-Acid Glycoprotein (a1AGP) 48T/96T Homo sapiens (Human)
117211 C反應蛋白(CRP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for C Reactive Protein (CRP) 48T/96T Bos taurus; Bovine (Cattle)
117212 內(nèi)皮型一氧化氮合酶(NOS3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3) 48T/96T Cavia (Guinea pig )
117213 內(nèi)皮型一氧化氮合酶(NOS3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3) 48T/96T Sus scrofa; Porcine (Pig)
117214 神經(jīng)型一氧化氮合酶()檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Nitric Oxide Synthase 1, Neuronal () 48T/96T Rattus norvegicus (Rat)
117215 神經(jīng)型一氧化氮合酶()檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Nitric Oxide Synthase 1, Neuronal () 48T/96T Mus musculus (Mouse)
117216 神經(jīng)型一氧化氮合酶()檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Nitric Oxide Synthase 1, Neuronal () 48T/96T Homo sapiens (Human)
117217 脂質(zhì)轉(zhuǎn)運蛋白(CETP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Cholesteryl Ester Transfer Protein (CETP) 48T/96T Homo sapiens (Human)
117218 血管緊張素原(AGT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Angiotensinogen (AGT) 48T/96T Mus musculus (Mouse)
117219 血管緊張素原(AGT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Angiotensinogen (AGT) 48T/96T Rattus norvegicus (Rat)
117220 血管緊張素原(AGT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Angiotensinogen (AGT) 48T/96T Homo sapiens (Human)
117221 60kD熱休克蛋白(HSPD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Heat Shock 60kD Protein 1, Chaperonin (HSPD1) 48T/96T Mus musculus (Mouse)
117222 60kD熱休克蛋白(HSPD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Heat Shock 60kD Protein 1, Chaperonin (HSPD1) 48T/96T Rattus norvegicus (Rat)
117223 60kD熱休克蛋白(HSPD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Heat Shock 60kD Protein 1, Chaperonin (HSPD1) 48T/96T Homo sapiens (Human)
117224 丙二醛(MDA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Malondialdehyde (MDA) 48T/96T General
117225 超氧化物歧化酶1(SOD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Superoxide Dismutase 1, Soluble (SOD1) 48T/96T Mus musculus (Mouse)
117226 超氧化物歧化酶1(SOD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Superoxide Dismutase 1, Soluble (SOD1) 48T/96T Rattus norvegicus (Rat)
117227 超氧化物歧化酶(SOD)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Superoxide Dismutases (SOD) 48T/96T Homo sapiens (Human)
117228 90kDa熱休克蛋白β1(HSP90β1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Heat Shock Protein 90kDa Beta 1 (HSP90b1) 48T/96T Mus musculus (Mouse)
117229 90kDa熱休克蛋白β1(HSP90β1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Heat Shock Protein 90kDa Beta 1 (HSP90b1) 48T/96T Rattus norvegicus (Rat)
117230 90kDa熱休克蛋白β1(HSP90β1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Heat Shock Protein 90kDa Beta 1 (HSP90b1) 48T/96T Homo sapiens (Human)
117231 干擾素γ(IFNγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Interferon Gamma (IFNg) 48T/96T Chicken (Gallus)
117232 白介素6(IL6)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Interleukin 6 (IL6) 48T/96T Chicken (Gallus)
117233 B-淋巴細胞激活抗原B7-2(LAB7-2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for B-Lymphocyte Activation Antigen B7-2 (LAB7-2) 48T/96T Mus musculus (Mouse)
117234 B-淋巴細胞激活抗原B7-2(LAB7-2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for B-Lymphocyte Activation Antigen B7-2 (LAB7-2) 48T/96T Rattus norvegicus (Rat)
117235 B-淋巴細胞激活抗原B7-2(LAB7-2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for B-Lymphocyte Activation Antigen B7-2 (LAB7-2) 48T/96T Homo sapiens (Human)
117236 低氧誘導因子1α(HIF1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) 48T/96T Mus musculus (Mouse)
117237 低氧誘導因子1α(HIF1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) 48T/96T Rattus norvegicus (Rat)
117238 低氧誘導因子1α(HIF1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) 48T/96T Homo sapiens (Human)
117239 胰淀素(IAPP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Islet Amyloid Polypeptide (IAPP) 48T/96T Mus musculus (Mouse)
117240 人腮腺炎病毒IgM ELISA試劑盒胰淀素(IAPP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Islet Amyloid Polypeptide (IAPP) 48T/96T Rattus norvegicus (Rat)
將抗原或抗體固定在過程稱為包被(coating)。換言之,包被即是抗原或抗體結合到固相載體表面的過程,脂類物質(zhì)無法與固相載體結合,可將其在有機溶劑(例如乙醇)中溶解后加入人腮腺炎病毒IgM ELISA試劑盒板孔中,開蓋置冰箱過夜或冷風吹干,待酒精揮發(fā)后,讓脂質(zhì)自然干固在固相表面。抗心磷脂抗體的ELISA試劑一般采用這種包被方式。
人腮腺炎病毒IgM ELISA試劑盒
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