美國ND公司ND Protein Precipitation Kit 返回列表頁

美國National Diagnostics公司

ND Protein Precipitation Kit ND 蛋白沉淀試劑盒  

• Recovery of All Proteins from Complex Mixtures
• Precipitates as Little as 100ng of BSA at 0.25µg/ml
• Milder than TCA
• More Reliable than Acetonitrile or Ammonium Sulfate

Catalog Number: EC-888
 

National Diagnostics公司的美國ND蛋白沉淀試劑盒，具有以下優(yōu)點(diǎn)：

1.從復(fù)雜的樣品中回收所有的蛋白

2.能夠從低至0.25µg/ml濃度的BSA蛋白樣品中回收100ng的微量蛋白

3.比TCA更加溫和

4.比乙腈和硫酸銨沉淀更加有效可靠

ND蛋白沉淀試劑盒沉淀過程溫和且易于操作。它可以保證在低的蛋白濃度下大量的回收溶液中的蛋白，其他方法中易丟失的蛋白也可以用該試劑盒沉淀回收。常規(guī)以及復(fù)雜的非常規(guī)的實驗都適用，真正做到了溫和性、簡便性和有效性。

 
The ND Protein Precipitation Kit is mild on samples and easy to use. It casts the finest net of any procedure, allowing the high yield collection of all proteins in solution, precipitating even the most dilute proteins. The ND Protein Precipitation Kit allows collection of proteins that would be missed by other methods.

Ideal for both routine and difficult work, the ND Protein Precipitation Kit stands alone among all methods. It offers the best combination of mildness, simplicity and effectiveness in protein precipitation.
 

High Yield Recovery

10ug BSA in 1ml vol and 50 ng of BSA in 200ul were prepared from stock and precipitated following kit instructions. 10ug and 50ng of BSA in 5 ul were prepared as controls. After running on a 12% gel, lanes 2 and 3 show control and recovered 10ug BSA. Lanes 5 and 6 show control and recovered 50ng BSA. Densitometry of 10ug BSA showed 91.9% recovery.

Simplicity of Method

Reagent A and Reagent B form a coprecipitate with the protein.
 

After a simple wash, the protein pellet is ready to be redissolved in desired buffer.