預(yù)裝Bio-Spin離心柱選擇指南
| Bio-Spin 6 | Micro Bio-Spin 6 | Bio-Spin 30 | Micro Bio-Spin 30 | |
| 預(yù)裝載體 | 級(jí) Bio-Gel P-6 凝膠 | 級(jí) Bio-Gel P-6 凝膠 | 級(jí) Bio-Gel P-30 凝膠 | 級(jí) Bio-Gel P-30 凝膠 |
| 平衡緩沖液 | SSC buffer* | 10 mM Tris, pH 7.4 or SSC buffer* | SSC buffer* | 10 mM Tris, pH 7.4 or SSC buffer* |
| 應(yīng)用 | Desalting and buffer exchange | Desalting and buffer exchange | Desalting; nucleotide and small molecule removal | DNA sequencing reaction mixtures （Tris）, and small molecule removal |
| 柱床體積 | 1.1 ml | 0.7 ml | 1.1 ml | 0.7 ml |
| 回收率和滯留率 | 90% recovery of 20 bases or bp, 99% retention of salts | 90% recovery of 20 bases or bp, 99% retention of salts | 95% recovery of 22 bases or bp, 98% retention of ddNTPs | 95% recovery of 22 bases or bp, 98% retention of ddNTPs |
| 分子量排阻限度，球狀蛋白 | 6,000 | 6,000 | 40,000 | 40,000 |
| 樣品體積 | 50–100 μl | 10–75 μl | 50–100 μl | 10–75 μl |
| 離心機(jī)類型 | 水平離心機(jī) | 微型離心機(jī) | 水平離心機(jī) | 微型離心機(jī) |
| 高壓滅菌 | Yes | Yes | Yes | Yes |
* 150 mM NaCl, 17.5 mM sodium citrate, pH 7.0
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