鹿內(nèi)皮型一氧化氮合成酶(eNOS)ELISA Kit |
保存條件:2-8℃
用 途:主要用于實驗室科研實驗。不得用于臨床使用。
保質(zhì)期:6個月。
鹿內(nèi)皮型一氧化氮合成酶(eNOS)ELISA Kit |
鹿內(nèi)皮型一氧化氮合成酶(eNOS)ELISA Kit |
以及ELISA試劑盒使用發(fā)表的文章等。請我公司的銷售技術(shù)人員。
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標(biāo)本的采集及保存
1.血清:全血標(biāo)本請于室溫放置2小時或4℃過夜后于1000g離心20分鐘,取上清即可檢測,或?qū)?biāo)本放于-20℃或-80℃保存,但應(yīng)避免反復(fù)凍融。
2.血漿:可用EDTA或肝素作為抗凝劑,標(biāo)本采集后30分鐘內(nèi)于2 - 8°C 1000 g離心15分鐘,或?qū)?biāo)本放于-20℃或-80℃保存,但應(yīng)避免反復(fù)凍融。
3.細胞培養(yǎng)物上清或其它生物標(biāo)本:1000g離心20分鐘,取上清即可檢測,或?qū)?biāo)本放于-20℃或-80℃保存,但應(yīng)避免反復(fù)凍融。
檢測方法:ELISA酶聯(lián)免疫(雙抗夾心法)
檢測標(biāo)本:血清、血漿、組織、細胞上清液、尿液等。
ELISA試劑盒的組成:
1.30倍濃縮洗滌液 20ml×1瓶 7.顯色劑B液 6ml×1/瓶
2.酶標(biāo)試劑 6ml×1瓶 8.標(biāo)準(zhǔn)品(96μg/L) 0.5ml×1瓶
3.酶標(biāo)包被板 12孔×8條 9.標(biāo)準(zhǔn)品稀釋液 1.5ml×1瓶
4.樣品稀釋液 6ml×1瓶 10.說明書 1份
5.顯色劑A液 6ml×1瓶 11.封板膜 2張
6.顯色劑B液 6ml×1/瓶 12.密封袋 1個
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